The role of adenosine 1a receptor signaling on GFR early after the induction of sepsis.
Am J Physiol Renal Physiol. 2018 May 1; 314(5):F788-F797.
Urine Exosome Isolation and Characterization.
Methods Mol Biol. 2017; 1641:413-423.
Quantification of Exosomes.
Journal of Cellular Physiology. 2017 Jul; 232(7): 1587-1590
Urine Exosomes: An Emerging Trove of Biomarkers.
Advances in Clinical Chemistry. 2017; 78: 103-122
Vasopressin Regulates Extracellular Vesicle Uptake by Kidney Collecting Duct Cells.
Journal of the American Society of Nephrology. 2016 Nov; 27(11):3345-3355.
Lipopolysaccharide-Induced CD300b Receptor Binding to Toll-like Receptor 4 Alters Signaling to Drive Cytokine Responses that Enhance Septic Shock.
Immunity. 2016 Jun 21; 44(6): 1365-78
Antagonism of scavenger receptor CD36 by 5A peptide prevents chronic kidney disease progression in mice independent of blood pressure regulation.
Kidney International. 2016 Apr; 89(4):809-22
TLR4 mutant mice are protected from renal fibrosis and chronic kidney disease progression.
Physiological Reports. 2015 Sep; 3(9). pii: e12558
Pulsed Focused Ultrasound Pretreatment Improves Mesenchymal Stem Cell Efficacy in Preventing and Rescuing Established Acute Kidney Injury In Mice.
Stem Cells. 2015 Apr; 33(4): 1241-53
Comparison of serum creatinine and serum cystatin C as biomarkers to detect sepsis-induced acute kidney injury and to predict mortality in CD-1 mice.
Am J Physiol Renal Physiol. 2014 Oct 15; 307(8):F939-48
Automated quantification of renal fibrosis with Sirius Red and polarization contrast microscopy.
Physiol Rep. 2014 Jul 1; [Epub ahead of print]
Bioactive exosomes: possibilities for diagnosis and management of bladder cancer.
J Urol. 2014 Aug; 192(2):297-8
Quantification of human urinary exosomes by nanoparticle tracking analysis.
J Physiol. 2013 Dec 1; 591(Pt 23):5833-42
Urinary exosomes: a reservoir for biomarker discovery and potential mediators of intrarenal signalling.
Proteomics. 2013 May; 13(10-11):1572-80
A role for solvents in the toxicity of agricultural organophosphorus pesticides.
Toxicology. 2012 Apr 11; 294(2-3):94-103
Identification and proteomic profiling of exosomes in human cerebrospinal fluid.
J Transl Med. 2012 Jan 5; 10:5
Exosomal transmission of functional aquaporin 2 in kidney cortical collecting duct cells.
J Physiol. 2011 Dec 15; 589(Pt 24):6119-27
Cyclophilin A is a damage-associated molecular pattern molecule that mediates acetaminophen-induced liver injury.
J Immunol. 2011 Sep 15; 187(6):3347-52
The application of mass-spectrometry-based protein biomarker discovery to theragnostics.
Br J Clin Pharmacol. 2010 Apr; 69(4):367-78
7-oxysterols modulate glucocorticoid activity in adipocytes through competition for 11beta-hydroxysteroid dehydrogenase type.
Endocrinology. 2008 Dec; 149(12):5909-18
Employment and Education
Visiting/Research Fellow (2012 - current)
My work involves developing novel diagnostics and therapies for the detection, grading, and treatment of acute kidney injury and chronic kidney disease. I have taken discoveries from in vitro and in vivo models through to pilot studies in humans working with clinical collaborators.
Recent projects include automated quantification of fibrosis in histology sections and analysis of blood pressure and glomerular filtration rate time series data.
Instructor (2015 - 2017)
Co-taught the BIOF309 Introduction to Python course. Developed the syllabus and taught the BIOF509 Machine Learning and Object-Oriented Programming with Python course.
PhD Student (2007 - 2012)
Centre for Cardiovascular Science, University of Edinburgh
The exosomal proteome as a source of biomarkers for human disease.
Supervisor: Dr James Dear
- Validated and implemented an exosome purification strategy from human urine
- Systematically investigated factors mediating the release of exosomes from an in vitro murine cortical collecting duct cell model
- Identified exosomes as being present in adult human cerebrospinal fluid (CSF)
- Investigated the urinary and CSF exosomal proteomes using tryptic digest and whole protein LC-MS/MS
MSc Student (2006 - 2007)
Cardiovascular Science Program, University of Edinburgh
Dissertation project: Endothelial cell microparticles as biomarkers of cell activation and dysfunction in sepsis.
Spring project: The Gap Junction Potentiating Peptide Rotigaptide Does Not Augment the EDHF Response in Rat Mesenteric Arteries.
Autumn project: Oxysterol exposure does not modulate 11β-HSD1 expression in 3T3-L1 adipocytes.
BSc Student (2002 - 2006)
Broad based degree in Biochemistry with the third year spent working in industry at GlaxoSmithKline.