Quantification of Exosomes.
Journal of Cellular Physiology. 2017 Jul; 232(7): 1587-1590
Urine Exosomes: An Emerging Trove of Biomarkers.
Advances in Clinical Chemistry 2017; 78: 103-122
Vasopressin Regulates Extracellular Vesicle Uptake by Kidney Collecting Duct Cells.
Lipopolysaccharide-Induced CD300b Receptor Binding to Toll-like Receptor 4 Alters Signaling to Drive Cytokine Responses that Enhance Septic Shock.
Immunity. 2016 Jun 21; 44(6): 1365-78
Antagonism of scavenger receptor CD36 by 5A peptide prevents chronic kidney disease progression in mice independent of blood pressure regulation.
Kidney International. 2016 Apr; 89(4):809-22
TLR4 mutant mice are protected from renal fibrosis and chronic kidney disease progression.
Physiological Reports. 2015 Sep; 3(9). pii: e12558
Pulsed Focused Ultrasound Pretreatment Improves Mesenchymal Stem Cell Efficacy in Preventing and Rescuing Established Acute Kidney Injury In Mice.
Stem Cells. 2015 Apr; 33(4): 1241-53
Comparison of serum creatinine and serum cystatin C as biomarkers to detect sepsis-induced acute kidney injury and to predict mortality in CD-1 mice.
Am J Physiol Renal Physiol. 2014 Oct 15; 307(8):F939-48
Automated quantification of renal fibrosis with Sirius Red and polarization contrast microscopy.
Physiol Rep. 2014 Jul 1; [Epub ahead of print]
Bioactive exosomes: possibilities for diagnosis and management of bladder cancer.
J Urol. 2014 Aug; 192(2):297-8
Quantification of human urinary exosomes by nanoparticle tracking analysis.
J Physiol. 2013 Dec 1; 591(Pt 23):5833-42
Urinary exosomes: a reservoir for biomarker discovery and potential mediators of intrarenal signalling.
Proteomics. 2013 May; 13(10-11):1572-80
A role for solvents in the toxicity of agricultural organophosphorus pesticides.
Toxicology. 2012 Apr 11; 294(2-3):94-103
Identification and proteomic profiling of exosomes in human cerebrospinal fluid.
J Transl Med. 2012 Jan 5; 10:5
Exosomal transmission of functional aquaporin 2 in kidney cortical collecting duct cells.
J Physiol. 2011 Dec 15; 589(Pt 24):6119-27
Cyclophilin A is a damage-associated molecular pattern molecule that mediates acetaminophen-induced liver injury.
J Immunol. 2011 Sep 15; 187(6):3347-52
The application of mass-spectrometry-based protein biomarker discovery to theragnostics.
Br J Clin Pharmacol. 2010 Apr; 69(4):367-78
7-oxysterols modulate glucocorticoid activity in adipocytes through competition for 11beta-hydroxysteroid dehydrogenase type.
Endocrinology. 2008 Dec; 149(12):5909-18
Employment and Education
Visiting Fellow (July 2012 - current)
Kidney Disease Branch, NIDDK, NIH
My work involves developing novel diagnostics and therapies for the detection, grading, and treatment of acute kidney injury and chronic kidney disease. I have taken discoveries from in vitro and in vivo models through to pilot studies in humans working with clinical collaborators.
PhD Student (2007 - 2012)
Centre for Cardiovascular Science, University of Edinburgh
The exosomal proteome as a source of biomarkers for human disease.
Supervisor: Dr James Dear
- Validated and implemented an exosome purification strategy from human urine
- Systematically investigated factors mediating the release of exosomes from an in vitro murine cortical collecting duct cell model
- Identified exosomes as being present in adult human cerebrospinal fluid (CSF)
- Investigated the urinary and CSF exosomal proteomes using tryptic digest and whole protein LC-MS/MS
MSc Student (2006 - 2007)
Cardiovascular Science Program, University of Edinburgh
Dissertation project: Endothelial cell microparticles as biomarkers of cell activation and dysfunction in sepsis.
Spring project: The Gap Junction Potentiating Peptide Rotigaptide Does Not Augment the EDHF Response in Rat Mesenteric Arteries.
Autumn project: Oxysterol exposure does not modulate 11β-HSD1 expression in 3T3-L1 adipocytes.
BSc Student (2002 - 2006)
Biochemistry Program, University of York
Broad based degree in Biochemistry with the third year spent working in industry at GlaxoSmithKline.